Functional role of epsilon-tubulin in the assembly of the centriolar microtubule scaffold

J Cell Biol. 2002 Sep 30;158(7):1183-93. doi: 10.1083/jcb.200205028.

Abstract

Centrioles and basal bodies fascinate by their spectacular architecture, featuring an arrangement of nine microtubule triplets into an axial symmetry, whose biogenesis relies on yet elusive mechanisms. However, the recent discovery of new tubulins, such as delta-, epsilon-, or eta-tubulin, could constitute a breakthrough for deciphering the assembly steps of this unconventional microtubule scaffold. Here, we report the functional analysis in vivo of epsilon-tubulin, based on gene silencing in Paramecium, which demonstrates that this protein, which localizes at the basal bodies, is essential for the assembly and anchorage of the centriolar microtubules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Cycle / physiology
  • Cell Division / physiology
  • Centrioles / metabolism*
  • Centrioles / physiology
  • Fluorescent Antibody Technique
  • Gene Silencing
  • Genes, Protozoan / genetics
  • Microinjections
  • Microtubules / physiology*
  • Microtubules / ultrastructure
  • Molecular Sequence Data
  • Paramecium / physiology*
  • Phenotype
  • Sequence Homology, Amino Acid
  • Tubulin / physiology*
  • Tubulin / ultrastructure

Substances

  • Tubulin