Abstract
Cucurbitacins 1 and 2 were isolated from the root of Trichosanthes kirilowii by tyrosinase inhibitory activity-guided fractionation. Spectroscopic analysis revealed that compounds 1 and 2 were cucurbitacin D and 23,24-dihydro-cucurbitacin D, respectively. Compounds 1 and 2 effectively inhibited the activity of tyrosinase (IC(50) = 0.18 microM and 6.7 microM, respectively), and the synthesis of melanin (IC(50) = 0.16 microM and 7.5 microM, respectively) in B16/F10 melanoma cells.
Publication types
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Letter
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / pharmacology*
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Inhibitory Concentration 50
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Melanins / antagonists & inhibitors*
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Melanins / biosynthesis
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Melanoma / metabolism
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Melanoma / pathology
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Mice
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Molecular Structure
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Monophenol Monooxygenase / antagonists & inhibitors*
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Plant Extracts / chemistry
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Plant Extracts / pharmacology
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Plant Roots / chemistry
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Trichosanthes*
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Triterpenes / chemistry
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Triterpenes / isolation & purification
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Triterpenes / pharmacology*
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Tumor Cells, Cultured / drug effects
Substances
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Antineoplastic Agents, Phytogenic
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Melanins
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Plant Extracts
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Triterpenes
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cucurbitacin D
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Monophenol Monooxygenase