Abstract
In a screen for proteins that interact with Jak2, we identified a previously uncharacterized 70-kDa protein and cloned the corresponding cDNA. The predicated sequence indicates that p70 contains an SH3 domain and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. p70 transcripts were found in all tissues examined. Similarly, when an antibody raised against a C-terminal peptide to analyze p70 protein expression was used, all murine tissues examined were found to express p70. To investigate the in vivo role of p70, we generated a p70-deficient mouse strain. Mice lacking p70 are viable, develop normally, and do not display any obvious abnormalities. No differences were detected in various hematological parameters, including bone marrow colony-forming ability, in response to cytokines that utilize Jak2. In addition, no impairment in B- and T-cell development and proliferative ability was detected.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Amino Acid Sequence
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Animals
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B-Lymphocytes / physiology
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Blotting, Western
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Bone Marrow Cells / cytology
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COS Cells
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Carrier Proteins / biosynthesis
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Carrier Proteins / chemistry*
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Carrier Proteins / genetics*
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Cell Division
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Cell Line
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Cell Separation
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Cloning, Molecular
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DNA, Complementary / metabolism
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Flow Cytometry
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Janus Kinase 2
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Mice
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Models, Genetic
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Molecular Sequence Data
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Open Reading Frames
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Peptides / metabolism
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Precipitin Tests
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Protein Binding
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Protein Structure, Tertiary
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Protein-Tyrosine Kinases / chemistry*
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Protein-Tyrosine Kinases / genetics*
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Proto-Oncogene Proteins*
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Signal Transduction
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T-Lymphocytes / physiology
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Tissue Distribution
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Transfection
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Tumor Cells, Cultured
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src Homology Domains
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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DNA, Complementary
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Peptides
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Proto-Oncogene Proteins
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RNA, Messenger
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p70 Jak2-interacting protein, mouse
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Protein-Tyrosine Kinases
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Jak2 protein, mouse
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Janus Kinase 2