Abstract
The arthropod-borne Toscana virus has been associated with acute neurological disease in humans. In this study, the viral envelope glycoproteins were expressed in soluble form in a baculovirus system. The recombinant sGN and sGC proteins were used as viral antigens in a Western blot assay to analyze the specific immune response in sera from patients with recognized virus-associated aseptic meningitis. The anti-glycoprotein and the anti-nucleoprotein N IgG responses were compared by an immunoassay based on the recombinant proteins. In this system, all the sera showed a high reactivity to the N protein, but they differed in the response to the glycoproteins.
Copyright 2002 Wiley-Liss, Inc.
MeSH terms
-
Animals
-
Antibodies, Viral / blood
-
Antibodies, Viral / immunology*
-
Antibody Specificity
-
Baculoviridae / genetics
-
Blotting, Western
-
Cell Line
-
Gene Expression Regulation, Viral
-
Genetic Vectors / genetics
-
Humans
-
Immune Sera / immunology
-
Molecular Sequence Data
-
Nucleocapsid Proteins / biosynthesis
-
Nucleocapsid Proteins / immunology
-
Phlebotomus Fever / diagnosis
-
Phlebotomus Fever / immunology*
-
Promoter Regions, Genetic
-
Recombinant Proteins / biosynthesis
-
Recombinant Proteins / immunology
-
Sandfly fever Naples virus / genetics
-
Sandfly fever Naples virus / immunology*
-
Spodoptera
-
Viral Fusion Proteins / biosynthesis
-
Viral Fusion Proteins / immunology*
Substances
-
Antibodies, Viral
-
Immune Sera
-
Nucleocapsid Proteins
-
Recombinant Proteins
-
Viral Fusion Proteins