Modulation of cell recruitment by anti-inflammatory agents in antigen-induced arthritis

Ann Rheum Dis. 2002 Nov;61(11):1027-30. doi: 10.1136/ard.61.11.1027.

Abstract

Objective: To study the effect of meloxicam (MXC) and diclofenac (DCF) on the recruitment of leucocytes during acute experimental arthritis.

Methods: Rabbits with antigen-induced arthritis were treated with MXC, DCF, or not treated. After 48 hours, synovial fluid (SF) leucocyte influx and prostaglandin E(2) (PGE(2)) levels were evaluated. Interleukin 8 (IL8) and monocyte chemotactic peptide-1 (MCP-1) expression and synthesis were studied in the inflamed tissues.

Results: Arthritic knees showed synovial effusion with a high leucocyte count and PGE(2) concentration, and an increased expression of IL8 and MCP-1. Both non-steroidal anti-inflammatory drugs (NSAIDs) reduced PGE(2) levels and the polymorphonuclear cell (PMN) concentration in SF, while the mononuclear cell (MN) concentration was unchanged in the treated groups in comparison with controls. A definite reduction of IL8 levels was obtained with the treatments, but the drugs did not prevent the up regulation of MCP-1.

Conclusion: The effect of these NSAIDs in acute arthritis may be related to the down regulation of IL8 production. The results suggest a differential effect of anti-inflammatory drugs on PMN and MN recruitment to the joint.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / therapeutic use*
  • Arthritis, Experimental / drug therapy*
  • Arthritis, Experimental / immunology
  • Chemokine CCL2 / genetics
  • Chemokine CCL2 / metabolism
  • Cyclooxygenase Inhibitors / therapeutic use
  • Diclofenac / therapeutic use*
  • Dinoprostone / biosynthesis
  • Gene Expression Regulation / drug effects
  • Interleukin-8 / genetics
  • Interleukin-8 / metabolism
  • Leukocytes / drug effects*
  • Leukocytes / immunology
  • Meloxicam
  • Neutrophil Infiltration / drug effects
  • Ovalbumin
  • Rabbits
  • Thiazines / therapeutic use*
  • Thiazoles / therapeutic use*

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Chemokine CCL2
  • Cyclooxygenase Inhibitors
  • Interleukin-8
  • Thiazines
  • Thiazoles
  • Diclofenac
  • Ovalbumin
  • Dinoprostone
  • Meloxicam