Abstract
The adenovirus early gene product Gam1 is crucial for virus replication and induces certain cellular genes by inactivating histone deacetylase 1 (HDAC1). We demonstrate that Gam1 (i) destroys promyelocitic leukemia nuclear bodies, (ii) delocalizes SUMO-1 into the cytoplasm and (iii) influences the SUMO-1 pathway. In addition, we show that Gam1 counteracts HDAC1 sumoylation both in vivo and in vitro. Sumoylation of HDAC1 does not seem to be absolutely required for HDAC1 biological activity but is part of a complex regulatory circuit that also includes phosphorylation of the deacetylase. Our data demonstrate that Gam1 is a viral protein that can affect simultaneously two signaling pathways: sumoylation and acetylation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
3T3 Cells
-
Adenoviridae / genetics
-
Adenoviridae / metabolism*
-
Animals
-
Cell Nucleus / metabolism*
-
Cytoplasm / metabolism
-
Gene Expression Regulation
-
HeLa Cells
-
Histone Deacetylase 1
-
Histone Deacetylases / genetics
-
Histone Deacetylases / metabolism*
-
Humans
-
Lysine / metabolism
-
Mice
-
Neoplasm Proteins / metabolism
-
Nuclear Proteins / metabolism
-
Promyelocytic Leukemia Protein
-
SUMO-1 Protein / metabolism*
-
Signal Transduction / physiology
-
Transcription Factors / metabolism
-
Tumor Suppressor Proteins
-
Viral Proteins / metabolism*
-
Virus Replication
Substances
-
CELO protein, adenovirus
-
Neoplasm Proteins
-
Nuclear Proteins
-
Pml protein, mouse
-
Promyelocytic Leukemia Protein
-
SUMO-1 Protein
-
Transcription Factors
-
Tumor Suppressor Proteins
-
Viral Proteins
-
PML protein, human
-
HDAC1 protein, human
-
Histone Deacetylase 1
-
Histone Deacetylases
-
Lysine