Abstract
In the present work, small interference RNA was used to knock-down acyl-CoA binding protein (ACBP) in HeLa, HepG2 and Chang cells. Transfection with ACBP-specific siRNA stopped growth, detached cells from the growth surface and blocked thymidine and acetate incorporation. The results show that depletion of ACBP in mammalian cells results in lethality, suggesting that ACBP is an essential protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetates / metabolism
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Apoptosis
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Blotting, Western
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Caspases / metabolism
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Cell Division
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Cell Line
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DNA Fragmentation
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Diazepam Binding Inhibitor / chemistry*
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Diazepam Binding Inhibitor / metabolism*
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Dose-Response Relationship, Drug
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Enzyme Activation
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HeLa Cells
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Humans
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Lipid Metabolism
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Microscopy, Phase-Contrast
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Protein Isoforms
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RNA, Double-Stranded / metabolism
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RNA, Small Interfering / metabolism
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Thymidine / metabolism
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Transfection
Substances
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Acetates
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Diazepam Binding Inhibitor
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Protein Isoforms
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RNA, Double-Stranded
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RNA, Small Interfering
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Caspases
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Thymidine