Synthesis and biological activity of strongly fluorescent tricyclic analogues of acyclovir and ganciclovir

J Med Chem. 2002 Nov 7;45(23):5052-7. doi: 10.1021/jm020827z.

Abstract

In search of strongly fluorescent tricyclic analogues of acyclovir (ACV, 1) and ganciclovir (GCV, 2), derivatives of the 3,9-dihydro-9-oxo-5H-imidazo[1,2-a]purine system, several 6-[4-(acyloxy)phenyl], 6-[4-(acylamino)phenyl], and 6-[4-(phenoxycarbonyloxy)phenyl]-substituted TACV and TGCV analogues were synthesized and evaluated for their activity against herpes simplex virus types 1 and 2 in cell culture. All TACV and TGCV analogues showed strong fluorescence (quantum yield of 30-65% vs 2-aminopurine 100%). The 6-[4-(phenoxycarbonyloxy)phenyl]-substituted compounds 11 and 19 displayed the best combination of the fluorescence and antiviral potency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclovir / analogs & derivatives*
  • Acyclovir / chemical synthesis*
  • Acyclovir / chemistry
  • Acyclovir / pharmacology
  • Antiviral Agents / chemical synthesis*
  • Antiviral Agents / chemistry
  • Antiviral Agents / pharmacology
  • Cell Culture Techniques
  • Fluorescent Dyes / chemical synthesis*
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / pharmacology
  • Ganciclovir / analogs & derivatives
  • Ganciclovir / chemical synthesis
  • Ganciclovir / chemistry
  • Ganciclovir / pharmacology
  • Herpesvirus 1, Human / drug effects
  • Herpesvirus 2, Human / drug effects
  • Heterocyclic Compounds, 3-Ring / chemical synthesis*
  • Heterocyclic Compounds, 3-Ring / chemistry
  • Heterocyclic Compounds, 3-Ring / pharmacology
  • Humans
  • Purines / chemical synthesis*
  • Purines / chemistry
  • Purines / pharmacology
  • Structure-Activity Relationship

Substances

  • 3,9-dihydro-3-((1,3-dihydroxy-2-propoxy)methyl)-9-oxo-6-(4-(phenoxycarbonyloxy)phenyl)-5H-imidazo(1,2-a)purine
  • 3,9-dihydro-3-((2-hydroxyethoxy)methyl)-9-oxo-6-(4-(phenoxycarbonyloxy)phenyl)-5H-imidazo(1,2-a)purine
  • Antiviral Agents
  • Fluorescent Dyes
  • Heterocyclic Compounds, 3-Ring
  • Purines
  • Ganciclovir
  • Acyclovir