Abstract
The gene encoding the green fluorescent protein (GFP) has been widely used in studies of gene expression. The GFP can be detected nondestructively in living cells or tissues by the green fluorescence of the protein under blue light. Solutions of enhanced GFP (EGFP) of known concentration were filled in glass capillaries and used to calibrate a method for quantitative determination of EGFP or GFP-S65T in plant cells. Images captured by a digital camera were analyzed to determine the linear range for measurement of EGFP expression. The value of the method was illustrated by analysis of the relative levels of GFP expression under control of different promoters in aleurone cells of barley.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / pharmacology
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Gene Expression Regulation, Bacterial
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Genes, Reporter / genetics
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Glass
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Green Fluorescent Proteins
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Hordeum / cytology
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Hordeum / genetics
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Hordeum / metabolism*
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Image Processing, Computer-Assisted / methods*
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Luminescent Proteins / analysis*
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Luminescent Proteins / biosynthesis
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Luminescent Proteins / genetics*
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Microscopy, Fluorescence
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Mutagenesis, Site-Directed
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plasmids
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Promoter Regions, Genetic / drug effects
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Promoter Regions, Genetic / genetics
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Seeds / cytology
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Seeds / genetics
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Time Factors
Substances
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Luminescent Proteins
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Plant Proteins
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Green Fluorescent Proteins
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Abscisic Acid