Abstract
It has recently been shown that adenoviral-mediated expression of peroxisome proliferator-activated receptor gamma co-activator-1 alpha (PGC-1 alpha) in hepatocytes stimulates glucose-6-phosphatase catalytic subunit (G6Pase) gene expression. A combination of fusion gene, gel retardation and chromatin immunoprecipitation assays revealed that, in H4IIE cells, PGC-1 alpha mediates this stimulation through an evolutionarily conserved region of the G6Pase promoter that binds hepatocyte nuclear factor-4 alpha.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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Binding Sites
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Catalytic Domain
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DNA Primers
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DNA-Binding Proteins*
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Gene Expression Regulation, Enzymologic
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Glucose-6-Phosphatase / chemistry
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Glucose-6-Phosphatase / genetics
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Glucose-6-Phosphatase / metabolism*
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Hepatocyte Nuclear Factor 4
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Mice
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Phosphoproteins / metabolism
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Phosphoproteins / physiology*
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Precipitin Tests
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Promoter Regions, Genetic
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Protein Binding
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Receptors, Cytoplasmic and Nuclear / metabolism
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Sequence Deletion
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Transcription Factors / metabolism
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Transcription Factors / physiology*
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Transcription, Genetic / physiology*
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Tumor Cells, Cultured
Substances
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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DNA Primers
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DNA-Binding Proteins
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Hepatocyte Nuclear Factor 4
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Phosphoproteins
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Receptors, Cytoplasmic and Nuclear
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Tcfl4 protein, mouse
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Transcription Factors
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peroxisome-proliferator-activated receptor-gamma coactivator-1
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Glucose-6-Phosphatase