Abstract
Objective:
To investigate the probabilities of core-biding factor a1 (Cbfa1) expression by human skin fibroblasts induced in vitro.
Methods:
The fibroblasts were isolated, purified from human skin, and were grown in incubation in the media of TNF-alpha, BMP-2, and combined TNF-alpha and BMP-2 at certain concentrations, respectively. The changes in biological features of these fibroblasts correlated with osteogenesis were detected by immunohistochemistry and RT-PCR assay.
Results:
TNF-alpha could switch phenotype of collagen in fibroblasts from Type I and III to Type I and induce fibroblasts to express Ras and BMP type I receptor (BMPR-IA). TNF-alpha in combination with BMP-2 could induce fibroblasts to express Cbfa1 and osteocalcin mRNA.
Conclusion:
Human skin fibroblast could be induced into pro-osteoblast expressing Cbfa1, an osteoblast-specific transcription factor and a regulation of osteoblast differentiation, and combined use of TNF-alpha and BMP-2 was one of the regulating factors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Protein Receptors, Type I
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Bone Morphogenetic Proteins / pharmacology
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Cells, Cultured
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Collagen / biosynthesis
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Core Binding Factor Alpha 1 Subunit
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Core Binding Factors
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Fibroblasts / metabolism
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Humans
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Neoplasm Proteins*
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Osteocalcin / biosynthesis
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Protein Serine-Threonine Kinases / biosynthesis
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RNA, Messenger / analysis
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Receptors, Growth Factor / biosynthesis
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Skin / cytology
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Transcription Factors / biosynthesis*
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Transcription Factors / genetics
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Transforming Growth Factor beta*
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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BMP2 protein, human
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Proteins
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Core Binding Factor Alpha 1 Subunit
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Core Binding Factors
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Neoplasm Proteins
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RNA, Messenger
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Receptors, Growth Factor
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Transcription Factors
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Transforming Growth Factor beta
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Tumor Necrosis Factor-alpha
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Osteocalcin
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Collagen
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Protein Serine-Threonine Kinases
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BMPR1A protein, human
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Bone Morphogenetic Protein Receptors, Type I