Abstract
We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 A(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen. The resulting enzyme, E-DreI (Engineered I-DmoI/I-CreI), binds a long chimeric DNA target site with nanomolar affinity, cleaving it precisely at a rate equivalent to its natural parents. The structure of an E-DreI/DNA complex demonstrates the accuracy of the protein interface redesign algorithm and reveals how catalytic function is maintained during the creation of the new endonuclease. These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Algorithms
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Base Sequence
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Binding Sites
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Catalysis
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Crystallography, X-Ray
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DNA / genetics
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DNA / metabolism
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DNA Restriction Enzymes / chemistry
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DNA Restriction Enzymes / genetics
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DNA Restriction Enzymes / metabolism
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Deoxyribonucleases, Type I Site-Specific / chemistry
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Deoxyribonucleases, Type I Site-Specific / genetics
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Deoxyribonucleases, Type I Site-Specific / metabolism
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Endonucleases / chemistry*
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Endonucleases / genetics
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Endonucleases / metabolism*
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Models, Molecular
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Protein Engineering / methods*
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Protein Folding
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Structure-Activity Relationship
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Substrate Specificity
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Thermodynamics
Substances
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Recombinant Fusion Proteins
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DNA
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Endonucleases
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DNA Restriction Enzymes
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endodeoxyribonuclease CreI
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endodeoxyribonuclease I-Dmo I
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Deoxyribonucleases, Type I Site-Specific