Abstract
The labeling of a monoclonal (anti-CEA) and a polyclonal (IgG) antibody with 153Sm has been investigated, using the bicyclic anhydride of DTPA (cDTPAa) as the chelating agent. The radiochemical study was performed using a combination of radioanalytical techniques (gel filtration, HPLC, ITLC-SG and SDS-PAGE). Optimization of factors affecting labeling (pH, Ab, Ab-DTPA concentration, etc.) leads to a labeling yield higher than 90%. Biodistribution studies in normal mice showed slow blood clearance and high uptake into the liver, kidney and lungs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / isolation & purification*
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Antibodies, Monoclonal / pharmacokinetics
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Antibodies, Monoclonal / therapeutic use
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Chelating Agents
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Chromatography, Gel
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Chromatography, High Pressure Liquid
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Female
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Immunoconjugates / isolation & purification*
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Immunoconjugates / pharmacokinetics
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Immunoconjugates / therapeutic use
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Mice
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Pentetic Acid / analogs & derivatives*
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Radiochemistry
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Radioimmunotherapy*
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Radioisotopes / isolation & purification*
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Radioisotopes / pharmacokinetics
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Radioisotopes / therapeutic use
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Samarium / isolation & purification*
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Samarium / pharmacokinetics
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Samarium / therapeutic use
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Tissue Distribution
Substances
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Antibodies, Monoclonal
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Chelating Agents
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Immunoconjugates
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Radioisotopes
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cyclic DTPA anhydride
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Samarium
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Pentetic Acid