Identification of neutralizing epitopes within structural domain III of the West Nile virus envelope protein

David W C Beasley; Alan D T Barrett

doi:10.1128/jvi.76.24.13097-13100.2002

Identification of neutralizing epitopes within structural domain III of the West Nile virus envelope protein

J Virol. 2002 Dec;76(24):13097-100. doi: 10.1128/jvi.76.24.13097-13100.2002.

Authors

David W C Beasley¹, Alan D T Barrett

Affiliation

¹ WHO Collaborating Center for Tropical Diseases, Sealy Center for Vaccine Development, Department of Pathology, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-0609, USA.

Abstract

Using a panel of neutralizing monoclonal antibodies, we have mapped epitopes in domain III of the envelope protein of the New York strain of West Nile virus. The ability of monoclonal antibodies that recognize these epitopes to neutralize virus appeared to differ between lineage I and II West Nile virus strains, and epitopes were located on the upper surface of domain III at residues E307, E330, and E332.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Antibodies, Monoclonal / immunology
Cell Membrane / metabolism
Epitope Mapping*
Mice
Neutralization Tests
Recombinant Proteins / immunology
Viral Envelope Proteins / immunology*
Viral Envelope Proteins / physiology
Virulence
West Nile virus / immunology*
West Nile virus / pathogenicity

Substances

Antibodies, Monoclonal
Recombinant Proteins
Viral Envelope Proteins