Abstract
To identify the roles of pyridoxal kinase (PLK) in epileptogenesis and the recovery mechanisms in spontaneous seizure, a chronological and comparative analysis of PLK expression in the gerbil hippocampus was conducted. PLK immunoreactivity in a pre-seizure group of seizure sensitive (SS) gerbils was more strongly detected than that in a seizure resistant (SR) group. The density of PLK immunoreactivity in a 30-min postictal group was significantly lower than that of a pre-seizure group. In a 12 h postictal group, PLK immunodensity recovered to pre-seizure level. The over-expression of PLK in the hippocampus of pre-seizure SS gerbils suggests that PLP play an important role in the modulation of GAD activity and GABA reuptake as mediated by membrane transporter via neurons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carrier Proteins / metabolism
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Cell Membrane / metabolism
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Dentate Gyrus / enzymology
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Dentate Gyrus / physiopathology
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Down-Regulation / physiology
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Epilepsy / enzymology*
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Epilepsy / physiopathology
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Fluorescent Antibody Technique
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GABA Plasma Membrane Transport Proteins
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Gerbillinae
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Glutamate Decarboxylase / metabolism*
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Hippocampus / enzymology*
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Hippocampus / physiopathology
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Immunohistochemistry
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Isoenzymes / metabolism
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Membrane Proteins / metabolism
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Membrane Transport Proteins*
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Neural Inhibition / physiology
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Neurons / enzymology*
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Organic Anion Transporters*
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Pyridoxal Kinase / metabolism*
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Reaction Time / physiology
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gamma-Aminobutyric Acid / metabolism*
Substances
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Carrier Proteins
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GABA Plasma Membrane Transport Proteins
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Isoenzymes
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Membrane Proteins
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Membrane Transport Proteins
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Organic Anion Transporters
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gamma-Aminobutyric Acid
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Pyridoxal Kinase
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Glutamate Decarboxylase
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glutamate decarboxylase 1
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glutamate decarboxylase 2