T cells of atomic bomb survivors respond poorly to stimulation by Staphylococcus aureus toxins in vitro: does this stem from their peripheral lymphocyte populations having a diminished naïve CD4 T-cell content?

Radiat Res. 2002 Dec;158(6):715-24. doi: 10.1667/0033-7587(2002)158[0715:tcoabs]2.0.co;2.

Abstract

We found previously that the peripheral CD4 T-cell populations of heavily exposed A-bomb survivors contained fewer naïve T cells than we detected in the corresponding unexposed controls. To determine whether this demonstrable impairment of the CD4 T-cell immunity of A-bomb survivors was likely to affect the responsiveness of their immune systems to infection by common pathogens, we tested the T cells of 723 survivors for their ability to proliferate in vitro after a challenge by each of the Staphylococcus aureus toxins SEB, SEC-2, SEC-3, SEE and TSST-1. The results presented here reveal that the proliferative responses of T cells of A-bomb survivors became progressively weaker as the radiation dose increased and did so in a manner that correlated well with the decreasing CD45RA-positive (naïve) [but not CD45RA-negative (memory)] CD4 T-cell percentages that we found in their peripheral blood lymphocyte (PBL) populations. We also noted that the T cells of survivors with a history of myocardial infarction tended to respond poorly to several (or even all) of the S. aureus toxins, and that these same individuals had proportionally fewer CD45RA-positive (naïve) CD4 T cells in their PBL populations than we detected in survivors with no myocardial infarction in their history. Taken together, these results clearly indicate that A-bomb irradiation led to an impairment of the ability of exposed individuals to maintain their naïve T-cell pools. This may explain why A-bomb survivors tend to respond poorly to toxins encoded by the common pathogenic bacterium S. aureus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Antibodies, Monoclonal / metabolism
  • Bacterial Toxins / pharmacology*
  • CD4 Lymphocyte Count*
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / radiation effects*
  • Cell Division / radiation effects
  • Dose-Response Relationship, Radiation
  • Female
  • Flow Cytometry
  • Humans
  • Japan
  • Leukocyte Common Antigens / biosynthesis
  • Logistic Models
  • Male
  • Middle Aged
  • Myocardial Infarction / blood
  • Nuclear Warfare*
  • Radioactive Fallout*
  • Staphylococcus aureus / metabolism
  • Survivors
  • T-Lymphocytes / radiation effects*

Substances

  • Antibodies, Monoclonal
  • Bacterial Toxins
  • Radioactive Fallout
  • Leukocyte Common Antigens