Abstract
ALL-1 is a member of the human trithorax/Polycomb gene family and is also involved in acute leukemia. ALL-1 is present within a stable, very large multiprotein supercomplex composed of > or =29 proteins. The majority of the latter are components of the human transcription complexes TFIID (including TBP), SWI/SNF, NuRD, hSNF2H, and Sin3A. Other components are involved in RNA processing or in histone methylation. The complex remodels, acetylates, deacetylates, and methylates nucleosomes and/or free histones. The complex's H3-K4 methylation activity is conferred by the ALL-1 SET domain. Chromatin immunoprecipitations show that ALL-1 and other complex components examined are bound at the promoter of an active ALL-1-dependent Hox a9 gene. In parallel, H3-K4 is methylated, and histones H3 and H4 are acetylated at this promoter.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Blotting, Western
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Cell Nucleus / metabolism
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Chromatin / metabolism
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism*
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HeLa Cells
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Histone Methyltransferases
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Histone-Lysine N-Methyltransferase*
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Histones / metabolism
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Homeodomain Proteins / metabolism
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Humans
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K562 Cells
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Mass Spectrometry
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Methylation
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Methyltransferases / chemistry*
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Methyltransferases / metabolism
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Myeloid-Lymphoid Leukemia Protein
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Precipitin Tests
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Protein Binding
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Protein Methyltransferases
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Protein Processing, Post-Translational
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Protein Structure, Tertiary
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Proto-Oncogenes*
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Silver Staining
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Transcription Factors*
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Transcription, Genetic*
Substances
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Chromatin
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DNA-Binding Proteins
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Histones
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Homeodomain Proteins
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KMT2A protein, human
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Transcription Factors
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homeobox protein HOXA9
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Myeloid-Lymphoid Leukemia Protein
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Histone Methyltransferases
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Methyltransferases
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Protein Methyltransferases
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Histone-Lysine N-Methyltransferase