Distinct regulation of internal ribosome entry site-mediated translation following cellular stress is mediated by apoptotic fragments of eIF4G translation initiation factor family members eIF4GI and p97/DAP5/NAT1

J Biol Chem. 2003 Feb 7;278(6):3572-9. doi: 10.1074/jbc.M206781200. Epub 2002 Nov 27.

Abstract

Many cellular stresses lead to the inhibition of protein synthesis. Despite this, some cellular mRNAs are selectively translated under these conditions. It was suggested that the presence of internal ribosome entry site (IRES) sequences in the 5'-untranslated regions allow these mRNAs to be actively translated despite the overall cessation of protein synthesis. Here we tested the hypothesis that the IRES elements of genes that are involved in the control of cell survival are distinctly regulated by cellular stresses. We show that the transient conditions of cellular stress favor the translation of pro-survival IRES, while the severe apoptotic conditions support translation of pro-death IRES elements. Furthermore, activation of pro-death IRES during the etoposide-induced apoptosis is caspase-dependent and correlates with the expression of apoptotic fragments of two members of the eIF4G translation initiation factor family, p97/DAP5/NAT1 and eIF4GI. Our results suggest that the regulation of IRES translation during stress contributes to the fine-tuning of cell fate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis*
  • Cell Line
  • Etoposide / pharmacology
  • Eukaryotic Initiation Factor-4G*
  • Humans
  • Oxidative Stress*
  • Peptide Fragments / physiology*
  • Peptide Initiation Factors / physiology*
  • Protein Biosynthesis / drug effects
  • Protein Biosynthesis / physiology*
  • RNA, Messenger / genetics
  • Ribosomes / metabolism*

Substances

  • EIF4G1 protein, human
  • EIF4G2 protein, human
  • Eukaryotic Initiation Factor-4G
  • Peptide Fragments
  • Peptide Initiation Factors
  • RNA, Messenger
  • Etoposide