Abstract
The central nervous system produces many of the enzymes responsible for corticosteroid synthesis. A model system to study the regulation of this local system would be valuable. Previously, we have shown that primary cultures of hippocampal neurons isolated from the fetal rat can perform the biochemical reactions associated with the enzymes 11beta-hydroxylase and aldosterone synthase. Here, we demonstrate directly that these enzymes are present within primary cultures of fetal rat hippocampal neurons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenal Glands / embryology
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Adrenal Glands / enzymology
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Animals
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Blotting, Western
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Cells, Cultured
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Cholesterol Side-Chain Cleavage Enzyme / genetics
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Cytochrome P-450 CYP11B2 / genetics
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Cytochrome P-450 CYP11B2 / metabolism*
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Enzymologic*
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Hippocampus / cytology
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Hippocampus / embryology*
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Hippocampus / enzymology*
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Neurons / enzymology*
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Phosphoproteins / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Rats, Wistar
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Reverse Transcriptase Polymerase Chain Reaction
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Steroid 11-beta-Hydroxylase / genetics
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Steroid 11-beta-Hydroxylase / metabolism*
Substances
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Phosphoproteins
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RNA, Messenger
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steroidogenic acute regulatory protein
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Cytochrome P-450 CYP11B2
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Steroid 11-beta-Hydroxylase
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Cholesterol Side-Chain Cleavage Enzyme