Abstract
A machine that employs a novel reagent delivery technique for biomolecular synthesis has been developed. This machine separates the addressing of individual synthesis sites from the actual process of reagent delivery by using masks placed over the sites. Because of this separation, this machine is both cost-effective and scalable, and thus the time required to synthesize 384 or 1536 unique biomolecules is very nearly the same. Importantly, the mask design allows scaling of the number of synthesis sites without the addition of new valving. Physical and biological comparisons between DNA made on a commercially available synthesizer and this unit show that it produces DNA of similar quality.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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DNA / biosynthesis
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DNA / chemical synthesis*
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DNA / economics
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Directed Molecular Evolution / economics
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Directed Molecular Evolution / instrumentation*
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Directed Molecular Evolution / methods*
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Indicators and Reagents
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Oligonucleotide Probes / biosynthesis
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Oligonucleotide Probes / chemical synthesis
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Oligonucleotide Probes / economics
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Polymerase Chain Reaction
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Sequence Analysis, DNA / economics
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Sequence Analysis, DNA / instrumentation
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Sequence Analysis, DNA / methods
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Software
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Indicators and Reagents
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Oligonucleotide Probes
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DNA