Analysis of far-red light-regulated genome expression profiles of phytochrome A pathway mutants in Arabidopsis

Plant J. 2002 Dec;32(5):723-33. doi: 10.1046/j.1365-313x.2002.01462.x.

Abstract

Phytochrome A (phyA) is the primary photoreceptor responsible for various far-red (FR) light-mediated responses. Previous studies have identified multiple phyA signaling mutants, including both positive and negative regulators of the phyA-mediated responses. How these defined intermediates act to mediate FR light responses is largely unknown. Here a cDNA microarray was used to examine effects of those mutations on the far-red light control of genome expression. Clustering analysis of the genome expression profiles supports the notion that phyA signaling may entail a network with multiple paths, controlling overlapping yet distinct sets of gene expression. FHY1, FAR1 and FHY3 most likely act upstream in the phyA signaling network, close to the phyA photoreceptor itself. FIN219, SPA1 and REP1 most likely act somewhere more downstream in the network and control the expression of smaller sets of genes. Further, this study also provides genomics evidence for the partial functional redundancy between FAR1 and FHY3. These two homologous proteins control the expression of a largely overlapping set of genes, and likely act closely together in the phyA-mediated FR light responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis / radiation effects*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant / radiation effects*
  • Genes, Plant / genetics
  • Genome, Plant*
  • Infrared Rays*
  • Mutation / genetics*
  • Oligonucleotide Array Sequence Analysis
  • Phytochrome / genetics
  • Phytochrome / metabolism*
  • Phytochrome A
  • Signal Transduction / radiation effects

Substances

  • Arabidopsis Proteins
  • PHYA protein, Arabidopsis
  • Phytochrome A
  • Phytochrome