HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sites

Nature. 2002 Dec 12;420(6916):678-82. doi: 10.1038/nature01188.

Abstract

The ability of human immunodeficiency virus (HIV-1) to persist and cause AIDS is dependent on its avoidance of antibody-mediated neutralization. The virus elicits abundant, envelope-directed antibodies that have little neutralization capacity. This lack of neutralization is paradoxical, given the functional conservation and exposure of receptor-binding sites on the gp120 envelope glycoprotein, which are larger than the typical antibody footprint and should therefore be accessible for antibody binding. Because gp120-receptor interactions involve conformational reorganization, we measured the entropies of binding for 20 gp120-reactive antibodies. Here we show that recognition by receptor-binding-site antibodies induces conformational change. Correlation with neutralization potency and analysis of receptor-antibody thermodynamic cycles suggested a receptor-binding-site 'conformational masking' mechanism of neutralization escape. To understand how such an escape mechanism would be compatible with virus-receptor interactions, we tested a soluble dodecameric receptor molecule and found that it neutralized primary HIV-1 isolates with great potency, showing that simultaneous binding of viral envelope glycoproteins by multiple receptors creates sufficient avidity to compensate for such masking. Because this solution is available for cell-surface receptors but not for most antibodies, conformational masking enables HIV-1 to maintain receptor binding and simultaneously to resist neutralization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibody Affinity
  • Binding Sites
  • CD4 Antigens / chemistry
  • CD4 Antigens / metabolism
  • Calorimetry
  • Entropy*
  • Epitopes / chemistry
  • Epitopes / immunology
  • Epitopes / metabolism
  • Glycosylation
  • HIV Antibodies / immunology*
  • HIV Envelope Protein gp120 / chemistry*
  • HIV Envelope Protein gp120 / immunology*
  • HIV Envelope Protein gp120 / metabolism
  • HIV-1 / chemistry*
  • HIV-1 / immunology*
  • HIV-1 / metabolism
  • Humans
  • Models, Molecular
  • Neutralization Tests
  • Protein Conformation
  • Receptors, HIV / chemistry
  • Receptors, HIV / metabolism*

Substances

  • CD4 Antigens
  • Epitopes
  • HIV Antibodies
  • HIV Envelope Protein gp120
  • Receptors, HIV