1,25-Dihydroxyvitamin D(3) increases striatal GDNF mRNA and protein expression in adult rats

Begoña Sanchez; Elena Lopez-Martin; Carmen Segura; Jose L Labandeira-Garcia; Roman Perez-Fernandez

doi:10.1016/s0169-328x(02)00545-4

1,25-Dihydroxyvitamin D(3) increases striatal GDNF mRNA and protein expression in adult rats

Brain Res Mol Brain Res. 2002 Dec;108(1-2):143-6. doi: 10.1016/s0169-328x(02)00545-4.

Authors

Begoña Sanchez¹, Elena Lopez-Martin, Carmen Segura, Jose L Labandeira-Garcia, Roman Perez-Fernandez

Affiliation

¹ Department of Physiology, School of Medicine, University of Santiago de Compostela, Spain.

PMID: 12480187
DOI: 10.1016/s0169-328x(02)00545-4

Abstract

Glial cell line-derived neurotrophic factor (GDNF) has been postulated as a possible candidate for therapeutic treatment in Parkinson's disease (PD). Recent in vitro data suggest that 1,25-dihydroxyvitamin D3 [1,25(OH)(2)D(3)] treatment may enhance GDNF mRNA expression. In the present study, using semiquantitative RT-PCR and Western blot, we have shown that 1,25(OH)(2)D(3) administration intraperitoneally, significantly increases GDNF mRNA and protein levels in the striatum of adult rats.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcitriol / pharmacology*
Calcium Channel Agonists / pharmacology*
Corpus Striatum / drug effects*
Corpus Striatum / physiology
Glial Cell Line-Derived Neurotrophic Factor
Humans
Nerve Growth Factors / genetics
Nerve Growth Factors / metabolism*
Parkinson Disease / metabolism
RNA, Messenger / metabolism*
Rats
Rats, Sprague-Dawley
Transcription, Genetic

Substances

Calcium Channel Agonists
GDNF protein, human
Gdnf protein, rat
Glial Cell Line-Derived Neurotrophic Factor
Nerve Growth Factors
RNA, Messenger
Calcitriol