Immobilized nicotinic receptor stationary phases: going with the flow in high-throughput screening and pharmacological studies

Ruin Moaddel; Irving W Wainer

doi:10.1016/s0731-7085(02)00513-7

Immobilized nicotinic receptor stationary phases: going with the flow in high-throughput screening and pharmacological studies

J Pharm Biomed Anal. 2003 Jan 15;30(6):1715-24. doi: 10.1016/s0731-7085(02)00513-7.

Authors

Ruin Moaddel¹, Irving W Wainer

Affiliation

¹ Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825, USA.

PMID: 12485712
DOI: 10.1016/s0731-7085(02)00513-7

Abstract

The nicotinic acetylcholine receptor (nAChR) subtypes alpha3beta4-nAChR and alpha4beta2-nAChR have been immobilized and the resulting stationary phases used to determine binding affinities. The alpha3beta4-nAChR column was coupled to a C(18) column and a mixture of 18 compounds was sorted into ligands and non-ligands for the alpha3beta4-nAChR. The results demonstrate that the nAChR stationary phases can be used for on-line high-throughput screening (HTS).

Publication types

Comparative Study
Review

MeSH terms

Animals
Chromatography, Affinity / instrumentation
Chromatography, Affinity / methods
Humans
Receptors, Nicotinic / analysis*
Receptors, Nicotinic / chemistry
Technology, Pharmaceutical / instrumentation
Technology, Pharmaceutical / methods*

Substances

Receptors, Nicotinic