Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance PCR and magnetic beads

Methods Mol Biol. 2002:192:99-108. doi: 10.1385/1-59259-177-9:099.
No abstract available

MeSH terms

  • Base Sequence
  • Cloning, Molecular / methods*
  • DNA Primers
  • DNA, Complementary / genetics
  • Indicators and Reagents
  • Magnetics
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction / methods*
  • RNA, Messenger / genetics
  • Sequence Analysis, DNA / methods
  • Templates, Genetic

Substances

  • DNA Primers
  • DNA, Complementary
  • Indicators and Reagents
  • RNA, Messenger