Enhanced immunogenicity to Mycobacterium tuberculosis by vaccination with an alphavirus plasmid replicon expressing antigen 85A

Infect Immun. 2003 Jan;71(1):575-9. doi: 10.1128/IAI.71.1.575-579.2003.

Abstract

The immunogenicity of a plasmid DNA vaccine incorporating Sindbis virus RNA replicase functions (pSINCP) and expressing antigen 85A (Ag85A) from Mycobacterium tuberculosis was compared with a conventional plasmid DNA vector encoding Ag85A. pSINCP-85A was highly immunogenic in mice and gave enhanced long-term protection against M. tuberculosis compared with the conventional vector.

Publication types

  • Comparative Study

MeSH terms

  • Acyltransferases / genetics
  • Acyltransferases / immunology*
  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Female
  • Interferon-gamma / biosynthesis
  • Mice
  • Mice, Inbred C57BL
  • Mycobacterium tuberculosis / immunology
  • Plasmids* / genetics
  • Plasmids* / immunology
  • RNA-Dependent RNA Polymerase / genetics
  • Replicon* / genetics
  • Replicon* / immunology
  • Sindbis Virus* / genetics
  • Sindbis Virus* / immunology
  • Tuberculosis Vaccines / administration & dosage
  • Tuberculosis Vaccines / immunology*
  • Tuberculosis, Pulmonary / prevention & control*
  • Vaccination
  • Vaccines, DNA / administration & dosage
  • Vaccines, DNA / immunology

Substances

  • Antigens, Bacterial
  • Tuberculosis Vaccines
  • Vaccines, DNA
  • Interferon-gamma
  • Acyltransferases
  • antigen 85A, Mycobacterium tuberculosis
  • RNA-Dependent RNA Polymerase