Spinning-disk confocal microscopy -- a cutting-edge tool for imaging of membrane traffic

Cell Struct Funct. 2002 Oct;27(5):349-55. doi: 10.1247/csf.27.349.

Abstract

Confocal laser scanning microscopy is experiencing a revolution in speed from the world of seconds to that of milliseconds. The spinning Nipkow disk method with microlenses has made this remarkable innovation possible. In combination with the ultrahigh-sensitivity, high-speed and high-resolution camera system based on avalanche multiplication of photoconduction, we are now able to observe the extremely dynamic movement of small vesicles in living cells in real time.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Eukaryotic Cells / cytology*
  • Eukaryotic Cells / metabolism
  • Golgi Apparatus / metabolism
  • Golgi Apparatus / ultrastructure
  • Humans
  • Image Processing, Computer-Assisted / instrumentation
  • Image Processing, Computer-Assisted / methods
  • Image Processing, Computer-Assisted / trends
  • Intracellular Membranes / metabolism
  • Intracellular Membranes / ultrastructure*
  • Lenses / standards
  • Lenses / trends
  • Microscopy, Confocal / instrumentation
  • Microscopy, Confocal / methods
  • Microscopy, Confocal / trends*
  • Microscopy, Video / instrumentation
  • Microscopy, Video / methods
  • Microscopy, Video / trends
  • Organelles / metabolism
  • Organelles / ultrastructure*
  • Transport Vesicles / metabolism
  • Transport Vesicles / ultrastructure*