Abstract
Confocal laser scanning microscopy is experiencing a revolution in speed from the world of seconds to that of milliseconds. The spinning Nipkow disk method with microlenses has made this remarkable innovation possible. In combination with the ultrahigh-sensitivity, high-speed and high-resolution camera system based on avalanche multiplication of photoconduction, we are now able to observe the extremely dynamic movement of small vesicles in living cells in real time.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Animals
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Eukaryotic Cells / cytology*
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Eukaryotic Cells / metabolism
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Golgi Apparatus / metabolism
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Golgi Apparatus / ultrastructure
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Humans
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Image Processing, Computer-Assisted / instrumentation
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Image Processing, Computer-Assisted / methods
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Image Processing, Computer-Assisted / trends
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Intracellular Membranes / metabolism
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Intracellular Membranes / ultrastructure*
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Lenses / standards
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Lenses / trends
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Microscopy, Confocal / instrumentation
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Microscopy, Confocal / methods
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Microscopy, Confocal / trends*
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Microscopy, Video / instrumentation
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Microscopy, Video / methods
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Microscopy, Video / trends
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Organelles / metabolism
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Organelles / ultrastructure*
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Transport Vesicles / metabolism
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Transport Vesicles / ultrastructure*