Express primer tool for high-throughput gene cloning and expression

Biotechniques. 2002 Dec;33(6):1328-33. doi: 10.2144/02336bc03.

Abstract

High-throughput approaches for gene cloning and expression require the development of new nonstandard tools for molecular biologists and biochemists. We introduce a Web-based tool to design primers specifically for the generation of expression clones for both laboratory-scale and high-throughput projects. The application is designed not only to allow the user complete flexibility to specify primer design parameters but also to minimize the amount of manual intervention needed to generate a large number of primers for the simultaneous amplification of multiple target genes.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Technical Report

MeSH terms

  • Algorithms
  • Base Composition
  • Base Sequence
  • Cloning, Molecular / methods*
  • DNA Primers* / chemical synthesis
  • DNA Primers* / chemistry
  • DNA, Bacterial / genetics
  • Drug Design*
  • Electronic Data Processing
  • Gene Expression
  • Gene Expression Profiling / methods*
  • Nucleic Acid Denaturation
  • Nucleic Acid Hybridization
  • Software*
  • Temperature

Substances

  • DNA Primers
  • DNA, Bacterial