Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a

Biochem Biophys Res Commun. 2003 Jan 10;300(2):297-304. doi: 10.1016/s0006-291x(02)02834-6.

Abstract

UFD2a is a mammalian homolog of Saccharomyces cerevisiae Ufd2, originally described as an E4 ubiquitination factor. UFD2a belongs to the U-box family of ubiquitin ligases (E3s) and likely functions as both an E3 and E4. We have isolated and characterized the mouse gene (Ube4b) for UFD2a. A full-length (approximately 5700 bp) Ube4b cDNA was isolated and the corresponding gene spans >100 kb, comprising 27 exons. Luciferase reporter gene analysis of the 5(') flanking region of Ube4b revealed that nucleotides -1018 to -943 (relative to the translation initiation site) possess promoter activity. This functional sequence contains two putative Sp1 binding sites but not a TATA box. Immunoblot and immunohistochemical analyses revealed that UFD2a is expressed predominantly in the neuronal tissues. We also show that UFD2a interacts with VCP (a AAA-family ATPase) that is thought to mediate protein folding. These data implicate UFD2a in the degradation of neuronal proteins by the ubiquitin-proteasome pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases
  • Animals
  • Base Sequence
  • Cell Cycle Proteins / metabolism
  • DNA, Complementary / isolation & purification
  • Fungal Proteins / genetics
  • Gene Components
  • Ligases / genetics*
  • Ligases / metabolism
  • Mice
  • Molecular Sequence Data
  • Neurons / metabolism
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae Proteins*
  • Transcriptional Activation
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitin-Protein Ligases
  • Valosin Containing Protein

Substances

  • Cell Cycle Proteins
  • DNA, Complementary
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Ubiquitin-Conjugating Enzymes
  • Ube4b protein, mouse
  • Ubiquitin-Protein Ligases
  • Adenosine Triphosphatases
  • CDC48 protein, S cerevisiae
  • Valosin Containing Protein
  • Vcp protein, mouse
  • Ligases
  • UFD2 protein, S cerevisiae

Associated data

  • GENBANK/AB083248
  • GENBANK/AB083249
  • GENBANK/AB083250
  • GENBANK/AB083251
  • GENBANK/AB083252
  • GENBANK/AB083253
  • GENBANK/AB083254
  • GENBANK/AB083255
  • GENBANK/AB083256
  • GENBANK/AB083257
  • GENBANK/AB083258
  • GENBANK/AB083259
  • GENBANK/AB083260
  • GENBANK/AB083261
  • GENBANK/AB083262
  • GENBANK/AB083263
  • GENBANK/AB083264
  • GENBANK/AB083265
  • GENBANK/AB083266
  • GENBANK/AB083267
  • GENBANK/AB083268
  • GENBANK/AB083269
  • GENBANK/AB083270
  • GENBANK/AB083271
  • GENBANK/AB083272
  • GENBANK/AB083273
  • GENBANK/AB083274