Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis

J Neuroimmunol. 2003 Jan;134(1-2):61-71. doi: 10.1016/s0165-5728(02)00401-0.

Abstract

We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Animals, Newborn
  • Cell Membrane / immunology
  • Cell Membrane / metabolism
  • Cell Movement / immunology*
  • Cells, Cultured
  • Central Nervous System / cytology
  • Central Nervous System / growth & development
  • Central Nervous System / metabolism*
  • Cerebellar Cortex / cytology
  • Cerebellar Cortex / growth & development
  • Cerebellar Cortex / metabolism
  • Chemokine CXCL1
  • Chemokine CXCL2
  • Chemokines / immunology*
  • Chemokines / metabolism
  • Chemokines, CXC*
  • Chemotactic Factors / immunology*
  • Chemotactic Factors / metabolism
  • Chemotaxis / immunology*
  • Excitatory Amino Acid Antagonists / pharmacology
  • Gene Expression Regulation, Developmental / physiology
  • Humans
  • Intercellular Signaling Peptides and Proteins / immunology*
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Neuroimmunomodulation / physiology
  • Neurons / cytology
  • Neurons / metabolism*
  • Precipitin Tests
  • Rats
  • Rats, Wistar
  • Receptors, AMPA / antagonists & inhibitors
  • Receptors, AMPA / genetics
  • Receptors, AMPA / immunology*
  • Receptors, AMPA / metabolism
  • Receptors, Interleukin-8B / immunology*
  • Receptors, Interleukin-8B / metabolism
  • Signal Transduction / immunology

Substances

  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokine CXCL2
  • Chemokines
  • Chemokines, CXC
  • Chemotactic Factors
  • Cxcl1 protein, rat
  • Cxcl2 protein, rat
  • Excitatory Amino Acid Antagonists
  • Intercellular Signaling Peptides and Proteins
  • Receptors, AMPA
  • Receptors, Interleukin-8B
  • glutamate receptor ionotropic, AMPA 1

Grants and funding