Abstract
Objective:
Investigating the bio-activities of peptides selected from phage display peptide library with vascular endothelial growth factor receptor Flt-1.
Methods:
Activities of DHFR-F56/F90 binding to human ubilial vein endothelial cells were detected by immunocytochemistry, and the activity of antiangiogenesis was determined with chick embryo chorioallantoric membrane (CAM) assay. Balb/c nude mice were used as model to detect the activity of DHFR-F56/F90 on inhibiting tumor growth, and immunohistochemistry was employed to determine the localization of the DHFR-F56/F90 in tumor.
Results:
DHFR-F56/F90 can bind to HUVEC, and DHFR-F56 inhibite angiogenesis in CAM. Meanwhile DHFR-F56 can bind with tumor cells, induce tumor necrosis and inhibit tumor growth in vivo.
Conclusion:
The peptide F56 is an effective antagonist of VEGF binding to Flt-1 and has a potent utility in antiangiogenesis and inhibiting tumor growth.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / pharmacology*
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Chick Embryo
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Endothelial Growth Factors / antagonists & inhibitors
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Immunohistochemistry
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Intercellular Signaling Peptides and Proteins
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Lymphokines / antagonists & inhibitors
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Mice
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Mice, Inbred BALB C
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Neovascularization, Physiologic / drug effects
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Peptide Library
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Peptides / metabolism
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Peptides / pharmacology*
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Recombinant Fusion Proteins / pharmacology*
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Tetrahydrofolate Dehydrogenase / pharmacology
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factor Receptor-1 / antagonists & inhibitors*
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Vascular Endothelial Growth Factor Receptor-1 / metabolism
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Vascular Endothelial Growth Factors
Substances
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Antineoplastic Agents
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Endothelial Growth Factors
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Intercellular Signaling Peptides and Proteins
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Lymphokines
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Peptide Library
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Peptides
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Recombinant Fusion Proteins
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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Tetrahydrofolate Dehydrogenase
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Vascular Endothelial Growth Factor Receptor-1