Abstract
To investigate the expression and function of the receptors of early-acting cytokines on cord blood CD34(+) hematopoietic stem/progenitor cells, the studies of Flt3 and c-kit were undertaken at the gene and protein levels. Fresh and cultured cord blood CD34(+) stem/progenitor cells were analyzed by flow cytometric two-color direct immunofluorescence methods and RT-PCR, while function of receptors was studied in vitro. It was found that (68.8 +/- 15.4)% of CD34(+) cells expressed Flt3, (50.6 +/- 12.7)% of CD34(+) cells expressed c-kit, the proportion of CD34(+) cells expressing Flt3 and c-kit decreased as in vitro culture time extended. It was concluded that cord blood CD34(+) stem/progenitor cells are capable of expressing Flt3 and c-kit for as long as 2 - 3 weeks in liquid medium, during the first week of culture, SCF and FL enhanced the generation of cells and progenitors notably.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD34 / blood*
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Cells, Cultured
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Fetal Blood / cytology*
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Flow Cytometry
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Hematopoietic Stem Cells / chemistry*
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Humans
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Proto-Oncogene Proteins / analysis*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / physiology
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Proto-Oncogene Proteins c-kit / analysis*
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Proto-Oncogene Proteins c-kit / genetics
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Proto-Oncogene Proteins c-kit / physiology
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RNA, Messenger / analysis
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Receptor Protein-Tyrosine Kinases / analysis*
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Receptor Protein-Tyrosine Kinases / genetics
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Receptor Protein-Tyrosine Kinases / physiology
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Reverse Transcriptase Polymerase Chain Reaction
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Stem Cell Factor / pharmacology
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fms-Like Tyrosine Kinase 3
Substances
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Antigens, CD34
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Proto-Oncogene Proteins
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RNA, Messenger
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Stem Cell Factor
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FLT3 protein, human
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Proto-Oncogene Proteins c-kit
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Receptor Protein-Tyrosine Kinases
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fms-Like Tyrosine Kinase 3