Abstract
A quantitative competitive PCR (QC-PCR) assay targeting the phlA gene of Pseudomonas fluorescens CHA0 was developed and tested in vitro. Statistically significant, positive correlations were found between QC-PCR and both CFU and total cell number when studying cells in log or stationary phase. The correlations disappeared when considering stressed cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Colony Count, Microbial
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Culture Media
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DNA, Bacterial / analysis
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Heat-Shock Response*
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Pest Control, Biological
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Phloroglucinol / analogs & derivatives
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Phloroglucinol / metabolism*
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Polymerase Chain Reaction / methods*
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Pseudomonas fluorescens / genetics
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Pseudomonas fluorescens / growth & development
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Pseudomonas fluorescens / metabolism
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Pseudomonas fluorescens / physiology*
Substances
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Bacterial Proteins
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Culture Media
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DNA, Bacterial
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Phloroglucinol