Abstract
The TGF-beta signals Nodal, Activin, GDF1, and Vg1 have been implicated in mesoderm induction and left-right patterning. Nodal and Activin both activate Activin receptors, but only Nodal requires EGF-CFC coreceptors for signaling. We report that Vg1 and GDF1 signaling in zebrafish also depends on EGF-CFC proteins, but not on Nodal signals. Correspondingly, we find that in Xenopus Vg1 and GDF1 bind to and signal through Activin receptors only in the presence of EGF-CFC proteins. These results establish that multiple TGF-beta signals converge on Activin receptor/EGF-CFC complexes and suggest a more widespread requirement for coreceptors in TGF-beta signaling than anticipated previously.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Activin Receptors, Type I / genetics
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Activin Receptors, Type I / physiology
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Activin Receptors, Type II / physiology*
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Animals
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Bone Morphogenetic Protein Receptors, Type I
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Bone Morphogenetic Proteins / physiology*
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Epidermal Growth Factor*
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GPI-Linked Proteins
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Glycoproteins / physiology*
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Growth Differentiation Factors
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Homeodomain Proteins / genetics
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Homeodomain Proteins / physiology*
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Intercellular Signaling Peptides and Proteins
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Macromolecular Substances
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Membrane Glycoproteins*
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Membrane Proteins
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Mice
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Neoplasm Proteins / physiology
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Nodal Protein
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Protein Serine-Threonine Kinases*
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Proteins*
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Receptors, Growth Factor*
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Receptors, Transforming Growth Factor beta / genetics
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Receptors, Transforming Growth Factor beta / physiology
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Recombinant Fusion Proteins / physiology
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Signal Transduction / physiology
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Species Specificity
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Transcription Factors / deficiency
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Transforming Growth Factor beta / physiology*
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Xenopus Proteins*
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Xenopus laevis / embryology
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Zebrafish
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Zebrafish Proteins*
Substances
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Bone Morphogenetic Proteins
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GDF1 protein, Xenopus
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GDF11 protein, human
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GPI-Linked Proteins
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Gdf11 protein, mouse
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Gdf3 protein, zebrafish
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Glycoproteins
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Growth Differentiation Factors
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Homeodomain Proteins
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Intercellular Signaling Peptides and Proteins
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Macromolecular Substances
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Membrane Glycoproteins
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Membrane Proteins
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Neoplasm Proteins
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Nodal Protein
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Nodal protein, mouse
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Proteins
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Receptors, Growth Factor
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Receptors, Transforming Growth Factor beta
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Recombinant Fusion Proteins
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Tdgf1 protein, mouse
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Transcription Factors
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Transforming Growth Factor beta
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Xenopus Proteins
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Zebrafish Proteins
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tdgf1 protein, zebrafish
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tdgf1.3 protein, Xenopus
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Epidermal Growth Factor
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Protein Serine-Threonine Kinases
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Activin Receptors, Type I
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Activin Receptors, Type II
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Bone Morphogenetic Protein Receptors, Type I
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activin receptor type II-B