[The experience of using the FTA-carriers for blood sample storage while conducting the molecular-genetic identification of unidentified killed persons--victims of military actions in the territory of the Chechen Republic]

Sud Med Ekspert. 2002 Nov-Dec;45(6):20-7.
[Article in Russian]

Abstract

The activity of adapting and introducing the FTA-carriers for the storage of blood samples of relatives of missing service men for the purpose of using them in the consequent identification measures in respect to non-identified killed persons has been conducted in the Russian Federation within the framework of identification of service men killed in the Chechen territory during the 1994-1996 armed conflict. The article generalizes the experience of practical usage of FTA-cards as an element in the technological sequence of the molecular-genetic identification expertise. Methods related with application of FTA-technologies were tested; the efficiency of polymerized chain reaction was evaluated by using the matrix of immobilized DNA for hypo variable segments HVS-1 and HVS-2 as well as for the D-loop of mitochondrial DNA and for chromosomal STR-locuses D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820.

Publication types

  • English Abstract

MeSH terms

  • Base Sequence
  • Blood Chemical Analysis / methods
  • Blood Chemical Analysis / standards*
  • Blood Specimen Collection / instrumentation*
  • Cadaver*
  • DNA Fingerprinting
  • Equipment Design
  • Forensic Medicine*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Russia
  • Warfare