Abstract
In the present paper, we report the identification of a new gene encoding a transmembrane protein of 520 amino acids, showing 22% amino acid identity with the extracellular domain of the interleukin (IL)-20 receptor. This gene, termed likely interleukin or cytokine receptor-2 ( LICR2 ), is located on chromosome 1, at 25 kb from the IL22R (IL-22 receptor) gene, and is constitutively expressed in most tissues. A chimaeric receptor, consisting of the extracellular domain of the IL-10 receptor alpha chain and the intracellular domain of LICR2, activated signal transducer and activator of transcription (STAT)1, STAT2, STAT3 and STAT5 upon IL-10 stimulation, in a Janus kinase 1-dependent manner. In contrast, none of the IL-10-related cytokines described so far could activate LICR2-transfected cells, suggesting that LICR2 is a signalling receptor for a new cytokine of the IL-10 family.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cloning, Molecular
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Humans
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Interleukin-10
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Janus Kinase 1
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Molecular Sequence Data
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Protein Structure, Tertiary
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Protein-Tyrosine Kinases / metabolism
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Receptors, Cytokine / genetics*
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Receptors, Cytokine / metabolism
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STAT1 Transcription Factor
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STAT2 Transcription Factor
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STAT3 Transcription Factor
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Signal Transduction / physiology
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transcription, Genetic / physiology
Substances
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DNA-Binding Proteins
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Receptors, Cytokine
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STAT1 Transcription Factor
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STAT1 protein, human
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STAT2 Transcription Factor
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STAT2 protein, human
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STAT3 Transcription Factor
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STAT3 protein, human
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Trans-Activators
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Interleukin-10
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Protein-Tyrosine Kinases
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JAK1 protein, human
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Janus Kinase 1
Associated data
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GENBANK/AJ543330
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GENBANK/AJ543331