A survey on the nutrition knowledge, attitude and behavior of 5145 persons from five cities of four provinces, Shandong, Guangdong, Sichuan and Heilongjiang, before and after the nutritional education project on "Dietary Guideline" has been effectively conducted. An obviously good effect has been achieved among the residents, middle and primary school students and elderly people. Before the nutritional educational project has been carried out, the people who understanding the "Dietary Guideline" was only 12.0%, 29.2% and 15.2% of residents, the elderly and students respectively. But after education, the rates raised to 93.4%, 99.0% and 91.9% respectively. Nearly 90% of residents and the elderly got to know the "Dietary Guideline" through the materials distributed from the project. The nutrition knowledge score was improved greatly after the project was conducted. The attitude towards learning nutrition knowledge was good. At the same time, some dietary behavior and the life style of people had been modified. These results indicated that nutrition education is very important on improving people's nutrition knowledge level, changing people's unhealthy living attitudes and dietary habits.