Abstract
The attenuated Erysipelothrix rhusiopathiae YS-19 strain was constructed for the purpose of delivering the C-terminal portion of the Mycoplasma hyopneumoniae P97 adhesin to the mucosal surface of the respiratory tract of pigs. In this study, the efficacy of the YS-19 vaccine against mycoplasmal pneumonia of swine was evaluated. Animal experiments revealed that intranasal immunization of pigs with the YS-19 strain significantly reduced the severity of pneumonic lung lesions caused by M. hyopneumoniae infection. In YS-19-immunized pigs, P97-specific serum antibodies were not detected. However, when stimulated with the P97 protein, peripheral blood mononuclear cells from the YS-19-immunized pigs had a significantly higher stimulation index (P<0.05) than that of cells from control pigs at 7 days post-challenge.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesins, Bacterial / immunology*
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Administration, Intranasal
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Animals
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Bacterial Vaccines / administration & dosage
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Bacterial Vaccines / immunology*
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Bronchoalveolar Lavage Fluid / immunology
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Bronchoalveolar Lavage Fluid / microbiology
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Enzyme-Linked Immunosorbent Assay
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Erysipelothrix / immunology*
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Erysipelothrix Infections / immunology*
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Erysipelothrix Infections / prevention & control*
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Immunity, Cellular / immunology
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Immunoglobulin A / biosynthesis
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Immunoglobulin A / immunology
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Immunoglobulin G / biosynthesis
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Immunoglobulin G / immunology
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Immunoglobulin M / biosynthesis
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Immunoglobulin M / immunology
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Lung / immunology
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Lung / microbiology
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Lung / pathology
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Mycoplasma / metabolism*
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Pneumonia of Swine, Mycoplasmal / immunology*
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Pneumonia of Swine, Mycoplasmal / prevention & control
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Pneumonia of Swine, Mycoplasmal / veterinary*
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Swine
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Swine Diseases / immunology*
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Swine Diseases / prevention & control
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Vaccines, Attenuated / immunology*
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / immunology*
Substances
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Adhesins, Bacterial
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Bacterial Vaccines
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Immunoglobulin A
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Immunoglobulin G
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Immunoglobulin M
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Vaccines, Attenuated
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Vaccines, Synthetic