Control of completeness and immediate detection of bleeding by a single laser-Doppler flow probe during intravascular middle cerebral artery occlusion in rats

J Neurosci Methods. 2002 Dec 31;122(1):75-8. doi: 10.1016/s0165-0270(02)00277-7.

Abstract

To detect the unintentional generation of subarachnoid hemorrhage (SAH) during thread occlusion of the middle cerebral artery (MCA) in rats, a single laser-Doppler flowmetry (LDF) probe was positioned over the ipsilateral cortex. Occlusion of the MCA resulted in an immediate drop of the LDF signal to 21.4+/-8.0% which was followed by a partial recovery to 31+/-11% in animals without SAH. In contrast, a sustained decrease to 7.4+/-5% (P<0.05 vs. non-SAH) occurred in animals with SAH. Thus, unintentional rupturing of the intracerebral carotid artery can easily and reliably be detected by single LDF monitoring immediately after MCA occlusion.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Blood Flow Velocity
  • Brain / blood supply
  • Brain / pathology
  • Disease Models, Animal
  • Hypoxia-Ischemia, Brain / etiology
  • Hypoxia-Ischemia, Brain / pathology*
  • Infarction, Middle Cerebral Artery / complications
  • Infarction, Middle Cerebral Artery / pathology*
  • Infarction, Middle Cerebral Artery / physiopathology*
  • Laser-Doppler Flowmetry / instrumentation*
  • Laser-Doppler Flowmetry / methods*
  • Male
  • Middle Cerebral Artery / physiopathology
  • Middle Cerebral Artery / surgery
  • Rats
  • Rats, Sprague-Dawley
  • Reference Values
  • Subarachnoid Hemorrhage / diagnosis*
  • Subarachnoid Hemorrhage / etiology
  • Subarachnoid Hemorrhage / physiopathology*