[Partial gene clone and nif gene homologous sequence analysis of Streptococcus sanguis]

Hua Xi Kou Qiang Yi Xue Za Zhi. 1999 Feb;17(1):11-3.
[Article in Chinese]

Abstract

Objective: To analyze the sequence of Streptococcus sanguis chromosome which contains one DNA fragment of 800 base pairs (bp) and discuss Streptococcus sanguis biological features of heredity.

Methods: Streptococcus DNA of 800-bp genetic fragment was cloned and analyzed by using eukaryotic expression vector.

Results: By Genbank database, it showed that the 800-bp genetic sequence was highly homologous with other bacterial nifS and nifU gene, and the highest homologous score was 114.

Conclusion: This nif gene of ATCC 10556 strain may correlate with nutrient metabolism and peroxide hydrogen release of Streptococcus sanguis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Genes, Bacterial*
  • Nitrogen Fixation / genetics
  • Sequence Homology, Amino Acid*
  • Streptococcus sanguis / genetics*