Abstract
An indirect enzyme-linked immunosorbent assay (ELISA) with Dicrocoelium dendriticum excretory/secretory antigens was used to evaluate the presence of serum antibodies against the trematode in 738 sheep randomly chosen in Sardinia (Italy). Coprological sedimentation was used to discover egg-output. Seropositivity was detected in 86.2% tested sheep, whereas faecal prevalence was 6.7%; all that were faecal-positive also were ELISA-positive.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Helminth / isolation & purification*
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Antigens, Helminth / immunology
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Dicrocoeliasis / diagnosis
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Dicrocoeliasis / immunology*
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Dicrocoeliasis / parasitology
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Dicrocoeliasis / veterinary*
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Dicrocoelium / immunology*
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Enzyme-Linked Immunosorbent Assay
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Feces / parasitology
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Female
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Italy
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Male
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Parasite Egg Count
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Prevalence
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Sensitivity and Specificity
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Sheep Diseases / diagnosis
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Sheep Diseases / immunology
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Sheep Diseases / parasitology
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Sheep, Domestic / immunology*
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Sheep, Domestic / parasitology*
Substances
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Antibodies, Helminth
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Antigens, Helminth