Abstract
DNAs encoding Trichosanthin, tobacco class I Chitinase and tobacco class I beta-1, 3-Glucanase were expressed in E. coli, respectively. The expression products were assayed for their anti-fungal activity. All of three kinds of proteins show anti-fungal activity. When two of them combined, this activity was enhanced greatly. When three of them combined, the stronger anti-fungal activity was observed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antifungal Agents / pharmacology*
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Chitinases / biosynthesis
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Chitinases / pharmacology*
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Drug Synergism
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Escherichia coli / genetics
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Fungi / drug effects*
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Glucan 1,3-beta-Glucosidase / biosynthesis
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Glucan 1,3-beta-Glucosidase / pharmacology*
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Nicotiana / enzymology
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
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Trichosanthin / biosynthesis
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Trichosanthin / pharmacology*
Substances
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Antifungal Agents
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Recombinant Proteins
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Trichosanthin
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Chitinases
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Glucan 1,3-beta-Glucosidase