[Sequence analysis of the mutated AOX2 promoter region in the Pichia pastoris]

Wei Sheng Wu Xue Bao. 1999 Dec;39(6):559-62.
[Article in Chinese]

Abstract

Spontaneous revertants of the AOX1-defective P. pastoris strain have been isolated, which were identified as phenotypically utilize methanol to grow as the wild type. The promoter region of the AOX2 gene from the revertant has been obtained by PCR amplification, and the DNA fragment is 1022 base pair in size. By the analysis of sequencing result and compared with the AOX2 gene sequences recorded in Gene Bank, two point mutations which at positions of -529 and -255(relative to the translation initiation codon) respectively, have been found. Since the positions are located in the AOX2 upstream repression sequences, the mutations may act interfere the repressor to combine with the functional sequences, and increase transcriptional activity. The above result implicated that the P. pastoris' ability to utilize methanol could be increased through modification the upper stream transcriptional regulation region in the AOX2 gene.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / genetics*
  • Base Sequence
  • Pichia / genetics*
  • Plasmids
  • Point Mutation*
  • Promoter Regions, Genetic

Substances

  • Alcohol Oxidoreductases
  • alcohol oxidase