[Nucleotide sequence analysis of the minimal replicon of the Streptomyces plasmid pSGL1]

H Zhang; B Hong; Y Li

[Nucleotide sequence analysis of the minimal replicon of the Streptomyces plasmid pSGL1]

Wei Sheng Wu Xue Bao. 1999 Aug;39(4):327-32.

[Article in Chinese]

Authors

H Zhang¹, B Hong, Y Li

Affiliation

¹ Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100050.

PMID: 12555571

Abstract

The high-copy-number plasmid pSGL1(7.4 kb) was isolated from Streptomyces globisporus. Deletion experiments showed its minimal replicon is located on the 2.0 kb Sau3 AI fragment. This fragment was subcloned. DNA sequence data analysis showed this fragment is a new sequence. Only an open reading frame with high coding probability is located on the minimal replicon. The deduced protein contains motifs characteristic of replicase for rolling-circle replication.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
DNA-Directed DNA Polymerase / chemistry
Molecular Sequence Data
Open Reading Frames
Plasmids / chemistry*
Replicon*
Streptomyces / genetics*

Substances

DNA replicase
DNA-Directed DNA Polymerase