Abstract
5' and 3' end sequence of acaBl gene as primers, the gene of halophilic thiolase from haloarchae, Halobacterium sp. ZP-6 was cloned and its amino acid composition was calculated. Compared with non-halophilic thiolase, the halophilic thiolase contains more negative charge amino acid, less positive amino acid and less strong hydrophobic amino acid, and use preferably small side-chain amino acid. Those suggest that electrostatic screen, hydrophobic effect and surface tension all contribute to halophilic properties of thiolase.
Publication types
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Comparative Study
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English Abstract
MeSH terms
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Acetyl-CoA C-Acetyltransferase / chemistry
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Acetyl-CoA C-Acetyltransferase / genetics*
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Acetyl-CoA C-Acetyltransferase / isolation & purification
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Amino Acid Sequence
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Amino Acids / analysis*
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Amino Acids / genetics
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Base Sequence
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Cloning, Molecular
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Halobacterium / enzymology*
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Halobacterium / genetics
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Halobacterium salinarum / enzymology
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Halobacterium salinarum / genetics
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Molecular Sequence Data
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Sequence Homology, Nucleic Acid
Substances
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Amino Acids
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Acetyl-CoA C-Acetyltransferase