The hemagglutinin (HA) gene from the AIV, A/Chicken/China/F/1998(H9N2), was amplified with the RT-PCR technique and directionally inserted into transferring vector 1175, resulted in recombinant transferring vector 1175HA. In order to generate recombinant fowlpox virus expressing HA(rFPV-HA), the recombinant transferring vector 1175HA was used to transfect the chicken embryo fibroblasts(CEF) pre-infected with wide type fowlpox virus. Then, by selection of blue plaques on the CEF overlaid with agar containing X-gal, rFPV-HA was obtained and purified. The expression of HA by rFPV-HA was detected in the recombinant virus-infected CEF by indirect immunofluorescence. Experiments on chickens demonstrated that rFPV-HA could induce detectable HI antibodies 7 days post-vaccination and those HI antibodies of relatively high titers could persist 55 days. rFPV-HA also had the same protective efficacies to suppress SPF chickens or commercial broiler chickens with antibodies against FPV from shedding challenged virus from intestine as inactivated vaccine in oil emulsion.