Alternative splicing of myeloid IgA Fc receptor (Fc alpha R, CD89) transcripts in inflammatory responses

FEBS Lett. 2003 Jan 30;535(1-3):205-9. doi: 10.1016/s0014-5793(02)03891-7.

Abstract

More than 10 splice variants of the Fc receptor for IgA (Fc alpha R, CD89) have been identified in human myeloid cells. In this study, we quantified Fc alpha R splice transcripts Delta EC2 and Delta 66 EC2, which lack the entire and a part of the homologous immunoglobulin-like extracellular domain 2 (EC2), respectively. Tumor necrosis factor-alpha was found to specifically increase the ratio of Delta EC2 to the wild type CD89 in neutrophils and conversely decrease the Delta EC2 ratio in monocytes. We also observed a significant decrease in the neutrophil Delta EC2/CD89 ratio in pneumonia patients. These results suggest that Delta EC2 is differentially regulated and could be involved in immunoregulation of IgA-mediated host defense.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing / drug effects
  • Alternative Splicing / genetics*
  • Alternative Splicing / immunology*
  • Antigens, CD / biosynthesis
  • Antigens, CD / genetics*
  • Antigens, CD / immunology*
  • Humans
  • Monocytes / drug effects
  • Monocytes / immunology
  • Myeloid Cells / drug effects
  • Myeloid Cells / immunology
  • Myeloid Cells / metabolism
  • Neutrophils / drug effects
  • Neutrophils / immunology
  • Pneumonia / immunology*
  • RNA, Messenger / metabolism
  • Receptors, Fc / biosynthesis
  • Receptors, Fc / genetics*
  • Receptors, Fc / immunology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Necrosis Factor-alpha / pharmacology
  • U937 Cells

Substances

  • Antigens, CD
  • Fc(alpha) receptor
  • RNA, Messenger
  • Receptors, Fc
  • Tumor Necrosis Factor-alpha
  • Tetradecanoylphorbol Acetate