Abstract
The detailed mechanism behind the processes of DNA-dependent RNA transcription initiation is largely unknown. When transcription initiation factors bind DNA, a significant change in the electrostatic state of the complex can result. Using electrical capacitance measurements of solutions of the YY1 zinc finger transcription initiation factor and the adeno-associated viral P5 promoter DNA, we observed a specific dielectric change when a protein-DNA complex was formed. We propose that complexation results in electrostatic changes that may trigger the markedly different electrical behavior, and offer a possible explanation for our results.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / genetics
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Animals
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DNA / chemistry*
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DNA / metabolism
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / metabolism
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Electric Capacitance
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Electrons*
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Erythroid-Specific DNA-Binding Factors
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Humans
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Promoter Regions, Genetic
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Protein Binding
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Recombinant Proteins
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Static Electricity
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Transcription Factors / chemistry*
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Transcription Factors / metabolism
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YY1 Transcription Factor
Substances
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DNA-Binding Proteins
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Erythroid-Specific DNA-Binding Factors
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Recombinant Proteins
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Transcription Factors
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YY1 Transcription Factor
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YY1 protein, human
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DNA