[Study on Methylation of p15(INK4B) Gene in Acute Myeloid Leukemia and Chronic Myeloid Leukemia]

Xiuzhi Guo; Hongtao Fan; Qiong Wu; Tao Zhou; Qiuye Guo; Peng Chen; Minhua Xu; Xueli Zhang; Gengxin Luo; Yang Xiao; Shi Liang

[Study on Methylation of p15(INK4B) Gene in Acute Myeloid Leukemia and Chronic Myeloid Leukemia]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2000 Dec;8(4):266-270.

[Article in Chinese]

Authors

Xiuzhi Guo¹, Hongtao Fan, Qiong Wu, Tao Zhou, Qiuye Guo, Peng Chen, Minhua Xu, Xueli Zhang, Gengxin Luo, Yang Xiao, Shi Liang

Affiliation

¹ Department of Hematology, Jinan University Medical School, Guangzhou 510632, China; Howard University, USA Department of Biochemistry, The First Military Medical University, Guangzhou 510515, China; Department of Hematotolgy, The First Affiliated Hospital, Jinan Univesity Medical School, Guangzhou 510632, China; Department of Hematology, Liuhuaqiao Hospital, Guangzhou 510010, China.

PMID: 12578663

Abstract

In order to explore the role of p15(INK4B) gene with highly methylated CpG island in the pathogenesis of leukemia, the expression levels of p15(INK4B) gene was detected in patients with AML and CML. Methylation-specific PCR (MSP) assay was employed in the experiments. The methylation incidence was 83.9% (26/31) in AML and 0% (0/28) in CML. The results showed that methylation of p15(INK4B) gene was the one of the major ways for inactivation of the gene, and the methylation could be appeared in clinical development of the disease and patients condition worsened. Methylation of p15(INK4B) did not occur and its function probably is normal in CML.