[The preliminary study of amniotic membrane on TGF-beta 1 expression after photorefractive keratectomy]

Yan Ke Xue Bao. 1999 Dec;15(4):215-7, 235.
[Article in Chinese]

Abstract

Objective: To investigate the effect of amniotic membrane on TGF-beta 1 expression after photorefractive keratectomy(PRK).

Methods: PRK was performed bilaterally on 6 New Zealand white rabbits. One eye was randomly tranplanted with preserved human amniotic membrane, and the other eye served as the control. The expression of TGF-beta 1 mRNA was detected with in situ hybridization at the 4th weeks after PRK.

Results: TGF-beta 1 mRNA expression was present in the corneal epithelium and stroma at the 4th weeks after PRK. The cornea in amniotic membrane transplanted group had also TGF-beta 1 mRNA expression, and the expression extent was less than that in control group (P < 0.05).

Conclusion: Amniotic membrane can reduce the expression of TGF-beta 1 mRNA in cornea after PRK.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amnion / transplantation*
  • Animals
  • Cornea / metabolism*
  • Cornea / surgery
  • Corneal Stroma / metabolism
  • Epithelium, Corneal / metabolism
  • Female
  • Humans
  • Lasers, Excimer
  • Male
  • Photorefractive Keratectomy*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rabbits
  • Random Allocation
  • Transforming Growth Factor beta / biosynthesis*
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta1

Substances

  • RNA, Messenger
  • TGFB1 protein, human
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1